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Blot, Southern: A test commonly used in molecular biology and genetics, the purpose of the test being to check for a match between DNA molecules.
In the Southern blot technique, DNA fragments are separated by a method called agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridized with complementary radiolabeled probes. The aim is to detect specific base sequences with the probes.
Lest all of this sound esoteric, note that in the television series "The X Files" a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person's genome).
The Southern blot is named after its inventor, the British biologist M.E. Southern. There is also a Northern blot and a Western blot.
Southern Blot Information. Learn about Southern blotting, the molecular biology protocol used to detect the presence of a specific DNA in a sample. Includes a detailed ...
This is a brief overview of how a Southern blot (more formally called an DNA blot) is performed and what type of data you can obtain form one.
The Southern Blot is one way to analyze the genetic patterns which appear in a person's DNA. Performing a Southern Blot involves: 1. Isolating the DNA in question from ...
The Southern blot is used to verify the presence or absence of a specific nucleotide sequence in the DNA from different sources and to identify the size of the ...
Southern Blot: Part 1. Making a DNA Blot. 1. Digest good-quality genomic or plasmid DNA to completion with restriction enzymes. Use AT LEAST 2 micrograms of genomic DNA ...