Hybridization, nucleic acid: A laboratory technique in which single-stranded nucleic acids (DNA or RNA) are allowed to interact so that complexes called hybrids are formed by molecules with similar, complementary sequences.
Through nucleic acid hybridization, the degree of sequence identity between nucleic acids can be determined and specific sequences detected in them. The hybridization can be carried out in solution or with one component immobilized on a gel or, most commonly, on nitrocellulose paper.
Hybrids are detected by various means: visualization in the electron microscope; by radioactively labelling one component and removing non-complexed DNA; or by washing or digestion with an enzyme that attacks single-stranded nucleic acids and finally estimating the radioactivity bound.
Hybridizations are done in all combinations: DNA-DNA (DNA can be rendered single-stranded by heat denaturation), DNA-RNA or RNA-RNA.
In situ hybridization involves hybridizing a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localization of genes to specific chromosomes via fluorescent in situ hybridization (FISH) analysis).
Nucleic acid hybridization is a fundamental tool in molecular genetics which takes advantage of the ability of individual single-stranded nucleic acid ...
Legend: Process of creating a hybrid strand of DNA/RNA. The two strands of a DNA molecule are denatured by heating to about 100Â°C = 212Â°F (a to b).
The second step is the actual hybridization of the probe to the filter bound nucleic acid. The following steps describe the Southern transfer procedure.
Nucleic acid hybridization: A technique in which single-stranded nucleic acids (DNA or RNA) are allowed to interact so that complexes called hybrids are formed by ...
DNA hybridization. Molecular medicine A technique for determining the presence of a target DNA in a sample of tissue or cells. See HLA analysis, Paternity testing ...